Endotoxin Gel-Clot Assay Kits

The R&D Systems Gel-Clot Endotoxin Assay Kit provides a reliable method for the visual detection of Gram-negative bacterial endotoxins (lipopolysaccharides) using the gel-clot formation technique. It utilizes Lyophilized Amebocyte Lysate (AL) Reagent derived from the circulating blood cells of the Asian horseshoe crab Tachypleus amebocyte.
 

When endotoxin is present, the serine protease proenzymes in the lysate are activated, converting coagulogen into coagulin and resulting in a gel-clot formation. If the endotoxin concentration meets or exceeds the assay's specified sensitivity (EU/mL), the gel remains intact after a 180° inversion of the reaction tube, indicating a positive result. Absence of gel-clot formation indicates that the endotoxin concentration is below the assays sensitivity threshold, signifying a negative result.