Endotoxin Kinetic Quantitation Kit

INTENDED USE AND DESCRIPTION

The R&D Systems™ Endotoxin Kinetic Chromogenic Quantitation Kit offers continuous, real-time monitoring of endotoxin-activated enzymatic reactions. This capability enables precise, sensitive, and reliable quantification, supporting stringent endotoxin control in pharmaceuticals, biologics, and laboratory products, and workflows. It provides improved accuracy for samples with variable turbidity or slow-reacting matrices and is particularly suited for complex sample types, applications requiring compliance-level quantitation, and workflows that demand low-level endotoxin detection with high reproducibility.

The kit includes a Lyophilized Amebocyte Lysate (AL) Reagent, sourced from the circulating amebocytes of the Asian horseshoe crab, Tachypleus tridentatus. In the presence of endotoxins, proenzymes in the lysate are activated to form coagulase enzymes, which catalyze the hydrolysis of a colorless substrate, releasing the yellow chromophore p-nitroaniline (pNA). The resulting color intensity is measured quantitatively at 405 nm, and the rate of color formation is directly correlated with the endotoxin concentration. A fast absorbance increase indicates a high endotoxin concentration, whereas a slow increase indicates a low concentration. Endotoxin levels are determined by comparing the sample reaction rate to a standard curve.

​R&D Systems Endotoxin Kinetic Quantitation Kit is designed for in vitro quantitative detection of Gram-negative bacterial endotoxins (Lipopolysaccharides) using a kinetic chromogenic method.

COMPONENTS

• Chromogenic Amebocyte Lysate (AL)
• Reconstitution Buffer
• Endotoxin Control Standard
• Endotoxin-free Water