Endotoxin rFC Quantitation Kit

INTENDED USE AND DESCRIPTION

The R&D Systems™ Endotoxin rFC Quantitation Kit provides an optimized, animal-free solution that meets regulatory expectations and ensures precise, highly sensitive, and reproducible endotoxin measurement. The kit uses recombinant expressed Tachypleus Factor C to leverage the endotoxin-activated enzymatic mechanism, where rFC detects endotoxin, becomes catalytically active, and cleaves a fluorescent substrate to generate a measurable signal directly proportional to endotoxin levels. Endotoxin concentrations in test samples are then determined by comparison to a standard curve.

R&D Systems rFC Endotoxin Test Kit enables in vitro quantitative measurement of gram-negative bacterial endotoxins (lipopolysaccharides) through activation of rFC.

Available in two assay ranges: 0.005-5 EU/mL (EFC005) and 0.01-10 EU/mL (EFC010).

Note: The R&D Systems rFC Endotoxin Test Kit (Recombinant Factor C Fluorometric Assay) contains sufficient reagents to perform 96 tests per kit.

COMPONENTS

• Recombinant Factor C (rFC)
• Reconsitution Buffer
• Endotoxin-free Water
• Endotoxin Control Standard
• Pyrogen-free Black 8-well Plate Strips