ErbB2/Her2 [p Tyr1221, p Tyr1222] Antibody (JE44-12)

Novus Biologicals | Catalog # NBP3-32769

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE44-12 expressed in HEK293
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding tyrosines 1221/1222 of human ErbB2/Her2 protein (Uniprot: P04626)

Modification

p Tyr 1221, p Tyr 1222

Localization

Cell membrane, Nucleus, Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

138 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ErbB2/Her2 [p Tyr1221, p Tyr1222] Antibody (JE44-12)

ErbB2/Her2 [p Tyr1221, p Tyr1222] Antibody (JE44-12)

Western Blot: ErbB2/Her2 [p Tyr1221, p Tyr1222] Antibody (JE44-12) [NBP3-32769]

Western Blot: ErbB2/Her2 [p Tyr1221, p Tyr1222] Antibody (JE44-12) [NBP3-32769] - Western blot analysis of ErbB2/Her2 on different lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32769) at 1/1,000 dilution.

Lane 1: SK-Br-3 whole cell lysate
Lane 2: SK-Br-3 treated with pp for 1 hour whole cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 138 kDa
Observed band size: 250 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32769) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for ErbB2/Her2 [p Tyr1221, p Tyr1222] Antibody (JE44-12)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ErbB2/Her2

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 [p Tyr1221, p Tyr1222] Antibody (JE44-12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ErbB2/Her2 [p Tyr1221, p Tyr1222] Antibody (JE44-12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for ErbB2/Her2 [p Tyr1221, p Tyr1222] Antibody (JE44-12)

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  • Q: I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?

    A:

    A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

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