Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all EVI2B Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Mouse EVI2B.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for EVI2B Antibody - Azide and BSA Free

Western Blot: EVI2B Antibody [NBP2-99056]

Western Blot: EVI2B Antibody [NBP2-99056]

Western Blot: EVI2B Antibody [NBP2-99056] - Lane A: Mouse heart tissue lysate Whole Cell LysateLane B: Mouse spleen tissue lysate Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:49 kDaObserved band size:49 kDa
Immunoprecipitation: EVI2B Antibody [NBP2-99056]

Immunoprecipitation: EVI2B Antibody [NBP2-99056]

Immunoprecipitation: EVI2B Antibody [NBP2-99056] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate1 uL anti-EVI2B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EVI2B rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 49 kDaObserved band size: 49 kDa

Applications for EVI2B Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EVI2B

The EVI2B gene encodes a 448 amino acid long, 48 kDA EVI2B protein that is frequently researched regarding its role in neurofibromatosis, leukemia, and myeloid leukemia.

Alternate Names

CD361 antigen, D17S376, ecotropic viral integration site 2B, Ecotropic viral integration site 2B protein homolog, EVDBprotein EVI2B, EVI-2B

Gene Symbol

EVI2B

Additional EVI2B Products

Product Documents for EVI2B Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EVI2B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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