Ficolins-1 (M-ficolin), -2, and -3 are secreted pattern-recognition lectins that contribute to the innate immune recognition of pathogens. Ficolins have one fibrinogen-like domain and one collagen-like domain and circulate as disulfide-linked homo-oligomers. Following ligand binding, ficolins associate with MASP serine proteases to trigger the lectin complement cascade.
Ficolin-3 Isoform 2 Antibody [Janelia Fluor® 525]
Novus Biologicals | Catalog # AF2367JF525
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Janelia Fluor 525
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Ficolin-3
Lys22-Arg299
Accession # O75636-1
Lys22-Arg299
Accession # O75636-1
Specificity
Detects human Ficolin-3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Ficolin-1 is observed and less 1% cross-reactivity with rhFicolin-2 and rhTenascinR is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Ficolin-3 Isoform 2 Antibody [Janelia Fluor® 525]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Ficolin-3
Long Name
Ficolin [Collagen/Fibrinogen Domain Containing] 3
Alternate Names
FCN3, FCNH, Ficolin3, H-Ficolin, HAKA1
Gene Symbol
FCN3
Additional Ficolin-3 Products
Product Documents for Ficolin-3 Isoform 2 Antibody [Janelia Fluor® 525]
Product Specific Notices for Ficolin-3 Isoform 2 Antibody [Janelia Fluor® 525]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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