Fluorescein Tyramide

Tocris Bioscience | Catalog # 6456

Green fluorescent reagent widely used for signal amplification in IHC and FISH
Tocris Bioscience

Key Product Details

Description

Green fluorescent reagent widely used for signal amplification in IHC and FISH

Wavelength

Excitation = 494 nm, Emission = 517 nm
View all Tyramide Signaling Amplification/TSA »

Product Description

Fluorescein Tyramide is a green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.

Optical Data

Fluorescein Tyramide Dye Spectra
Emission Color Green
λabs 494 nm
λem 517 nm
Extinction Coefficient (ε) 70000 M-1cm-1
Closest Laser line 488 nm
Application Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Product Specifications for Fluorescein Tyramide

Molecular Weight

495.48

Formula

C29H21NO7

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

3',6'-Dihydroxy-N-(4-hydroxyphenylethyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5(6)-carboxamide

CAS Number

210236-90-1

InChI Key

MJQMGAFVBOOBIB-UHFFFAOYSA-N

SMILES

OC1=CC2=C(C3(C4=CC=C(C=C4O2)O)C5=CC=C(C(NCCC6=CC=C(C=C6)O)=O)C=C5C(O3)=O)C=C1.OC7=CC8=C(C9(C%10=CC=C(C=C%10O8)O)C%11=CC(C(NCCC%12=CC=C(C=C%12)O)=O)=CC=C%11C(O9)=O)C=C7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 11.89 24

Preparing Stock Solutions for Fluorescein Tyramide

The following data is based on the product molecular weight 495.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.24 mM 8.41 mL 42.05 mL 84.09 mL
1.2 mM 1.68 mL 8.41 mL 16.82 mL
2.4 mM 0.84 mL 4.20 mL 8.41 mL
12 mM 0.17 mL 0.84 mL 1.68 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 4 publications citing the usage of this product.

Product Documents for Fluorescein Tyramide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fluorescein Tyramide

For research use only

Citations for Fluorescein Tyramide

Customer Reviews for Fluorescein Tyramide

There are currently no reviews for this product. Be the first to review Fluorescein Tyramide and earn rewards!

Have you used Fluorescein Tyramide?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card