g-linked ATP agarose resin

A: The binding of any ATP-binding protein to our resin will depend on a number of factors. These include amongst others: the formulation of the binding buffer, the incubation time of the sample with the resin (flow rate), the concentration of the ATP-binding protein, the affinity of the binding protein for ATP and the size of the binding protein (steric hindrance can be a factor with larger proteins). The only true way to establish the capacity of the column for any individual binding protein is by experimentation. If you require mg scale purification then our ATP agarose should be suitable, especially as the resin can be reused. The unique nature of our ATP resin is that it is not subject to dephosphorylation like many other similar resins available from other suppliers. This means that you can simply wash, re-equilibrate and reuse the resin multiple times. This will enable you to obtain mg quantities of your protein even if it takes more than one purification.