![Western Blot: G6PC Overexpression Lysate [NBP2-09053]](https://resources.rndsystems.com/images/products/G6PC-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09053-img0001.jpg "Western Blot: G6PC Overexpression Lysate [NBP2-09053]")
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Species
Applications
Product Summary for G6PC Overexpression Lysate
G6PC Transient Overexpression Lysate
Expression Host: HEK293T
Plasmid: RC215623
Accession#: NM_000151
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Product Specifications
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Type
Scientific Data Images for G6PC Overexpression Lysate
Western Blot: G6PC Overexpression Lysate [NBP2-09053]
Western Blot: G6PC Overexpression Lysate (Adult Normal) [NBP2-09053] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for G6PC.Formulation, Preparation, and Storage
Formulation
Concentration
Shipping
Storage
Background: G6PC
Alternate Names
Gene Symbol
Additional G6PC Products
Product Documents for G6PC Overexpression Lysate
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for G6PC Overexpression Lysate
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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Application: Western BlotSample Tested: Human Neuroblastoma Cell LinesSpecies: HumanVerified Customer | Posted 10/15/2014G6PC Levels in NB
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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FAQs for G6PC Overexpression Lysate
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Q: I was wondering if glucose 6 phosphatase has any phosphatase activity? I am interested in the dephosphorylation of mannose phosphate.
A: NBP2-09053 is an overexpression lysate of G6PC overexpressed in 293T cells. It is unpurified and has never been tested for activity. It is mainly intended to be used as a positive control.