GABA-A R alpha 6 C-Terminus Antibody

Catalog #: PPS028 Datasheet / COA / SDS

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PPS028

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Detection of GABAAR alpha 6 C-Terminus by Western Blot.

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GABA-A R alpha 6 C-Terminus Antibody Summary

Species Reactivity

Human, Mouse, Rat, Bovine

Specificity

Human, mouse, rat, and bovine ~57 kDa GABA_A R alpha 6 subunit, C-Terminus

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Fusion protein from the cytosolic loop of the rat GABA_A R alpha 6 subunit, C-Terminus

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of GABA_AR alpha 6 C-Terminus by Western Blot. Western blot of forebrain lysates from wild type and alpha 6 knockout animals, using approximately 5 - 7 μg of tissue per lane. Blots were incubated overnight at 2 - 8° C with anti-GABA_AR, alpha 6 subunit, C-Terminus diluted 1:1000. The antibody labeled the ~57 kDa alpha 6 subunit of the GABA_AR in the wild type (control) but not in the alpha 6 knockout animals.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R alpha 6

GABA_A ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABA_A receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABA_A receptors have some combination of alpha, beta and gamma subunits. The rat alpha 6 isoform is a 57 kDa, 434 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 14 - 221). The expression of the subunit is strongly associated with cerebellar granule cells. The alpha 6 subunit has also been linked to alcohol sensitivity, possible due to a point mutation substitution of arginine for a glutamine at amino acid position 81 in the extracellular N-terminus.

References

Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
Korpi, E.R. et al. (1993) Nature 361:356.
Luddens, H. et al. (1990) Nature 346:648.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-6 Polypeptide

Entrez Gene IDs

2559 (Human); 14399 (Mouse); 29708 (Rat)

Alternate Names

GABA A R alpha 6; GABA subunit A receptor alpha 6; GABA(A) receptor subunit alpha-6; GABAAR alpha 6; GABAARa6; GABRA6; gamma-aminobutyric acid (GABA) A receptor, alpha 6; gamma-aminobutyric acid receptor subunit alpha-6; MGC116903; MGC116904