Recombinant Human gamma C Crystallin His Protein

Novus Biologicals | Catalog # NBP1-98998

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all gamma C Crystallin Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-174 of Human gamma C Crystallin

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGKITF YEDRAFQGRS YETTTDCPNL QPYFSRCNSI RVESGCWMLY ERPNYQGQQY LLRRGEYPDY QQWMGLSDSI RSCCLIPQTV SHRLRLYERE DHKGLMMELS EDCPSIQDRF HLSEIRSLHV LEGCWVLYEL PNYRGRQYLL RPQEYRRCQD WGAMDAKAGS LRRVVDLY

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

23.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human gamma C Crystallin His Protein

SDS-PAGE: Recombinant Human gamma C Crystallin His Protein [NBP1-98998]

SDS-PAGE: Recombinant Human gamma C Crystallin His Protein [NBP1-98998]

SDS-Page: gamma C Crystallin Recombinant Protein [NBP1-98998]

Formulation, Preparation, and Storage

NBP1-98998
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 2 mM DTT, 200 mM NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: gamma C Crystallin

Gamma-crystallin C, also known as CRYGC, belongs to the beta/gamma-crystallin family. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. Defects in CRYGC are a cause of cataract autosomal dominant (ADC) and cataract Coppock-like (CCL). Recombinant human CRYGC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CCL, CRYG3gamma-C-crystallin, crystallin, gamma C, crystallin, gamma-3, Gamma-C-crystallin, Gamma-crystallin 2-1, Gamma-crystallin 3, gamma-crystallin C

Gene Symbol

CRYGC

Additional gamma C Crystallin Products

Product Documents for Recombinant Human gamma C Crystallin His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human gamma C Crystallin His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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