GAPDH primer

Novus Biologicals | Catalog # NBP1-71650

Novus Biologicals
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Key Product Details

Species

Human

Applications

Chromatin Immunoprecipitation

Product Specifications

Applications

Chromatin Immunoprecipitation (ChIP) (0.5 uM)

Application Notes

These GAPDH primers are useful for PCR in chromatin precipitation. Amplicon size for this primer set is 165 base pairs.

Scientific Data Images for GAPDH primer

Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.

Formulation, Preparation, and Storage

Formulation

10 uM in TE buffer.

Preservative

No Preservative

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GAPDH primer

Primers are designed to amplify DNA in exon 1 of the human GAPDH gene locus. GAPDH is an actively transcribed housekeeping gene with significant expression in all cell types. GAPDH is associated with modified histones targeted to active genes.

Additional GAPDH primer Products

Product Documents for GAPDH primer

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for GAPDH primer

This GAPDH primer contains both forward and reverser primers.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GAPDH primer

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  • Q: Do you have a Tm available for this primer set?

    A: The Tm for our GAPDH primer set is between 57.2-63.3 degrees C.

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