Recombinant Rat GDNF Protein

Novus Biologicals | Catalog # NBP2-35204

Novus Biologicals
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Key Product Details

Source

E. coli

Accession Number

Q07731

Applications

Bioactivity, SDS-PAGE
View all GDNF Proteins and Enzymes »

Product Specifications

Description

A homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains corresponding to GDNF Source: E. coli

Uniprot ID: Q07731

Amino Acid Sequence: SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of GDNF as determined by LAL method.

Predicted Molecular Mass

29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

GDNF protein is fully biologically active when compared to standard. Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. The ED50 for this effect is typically 1 - 3 ng/mL

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP2-35204
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDNF

Glial cell-derived neurotrophic factor is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha 1. It is a small protein that potently promotes the survival and morphological differentiation of various neuronal. It may also modulate local neuronal effects in distal regions of the motor neuron. GDNF Recombinant rat GDNF (monomer) contains 135 amino acids residues, which is a disulfide-linked homodimer and it shares 99 % and 93 % a.a. sequence identity with mouse and human GDNF.

Long Name

Glial Cell line-derived Growth Factor

Alternate Names

ATF, HFB1-GDNF, HGDNF, HSCR3

Gene Symbol

GDNF

UniProt

Q07731

Additional GDNF Products

Product Documents for Recombinant Rat GDNF Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Rat GDNF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

GDNF Family

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