Glucose Uptake Assay Kit (Fluorometric)

Cells

0.02 nmol/ul

0.02-0.3 nmol/ul

Quantitative

Assay Kit (Fluorometric)

Non-species specific

Detection principle: 2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm.

Detection method: Fluorescence method

Other instruments required: Micropipettor, Incubator, Water bath

Other reagents required: KRPH solution (20 mM Hepes, 5 mM KH2PO4, 1 mM MgSO4, 1 mM CaCl2, 136 mM NaCl, 4.7 mM KCl, 2% BSA, pH 7.4)

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage of components varies. See protocol for specific instructions.