Glutathione S-Transferase/GST Antibody (PIP-1D7-54.3)
R&D Systems | Catalog # MAB94991
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Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Specificity
Clonality
Host
Isotype
Scientific Data Images for Glutathione S-Transferase/GST Antibody (PIP-1D7-54.3)
Detection of Glutathione S‑Transferase/GST by Western Blot.
Western blot shows lysates ofSf 21 S. frugiperdainsect ovarian cell line mock transfected or transfected GST tagged Parkin. PVDF membrane was probed with 1 µg/mL of Hamster Anti-Glutathione S‑Transferase/GST Monoclonal Antibody (Catalog # MAB94991) followed by HRP-conjugated Anti-Hamster IgG Secondary Antibody. A specific band was detected for Glutathione S‑Transferase/GST tagged Parkin at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Glutathione S‑Transferase/GST by Western Blot.
Western Blot shows lysates of E. coli transfected with GST tagged BRDG1-SH2. PVDF membrane was probed with 0.5 µg/ml of Hamster Anti-Glutathione S‑Transferase/GST Monoclonal Antibody (Catalog # MAB94991) followed by HRP-conjugated Anti-Hamster IgG Secondary Antibody. A specific band was detected for Glutathione S‑Transferase/GST at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Glutathione S-Transferase/GST Antibody (PIP-1D7-54.3)
Western Blot
Sample: Sf 21 S. frugiperda insect ovarian cell line transfected GST tagged Parkin; E. coli transfected with GST tagged BRDG1-SH2
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Glutathione S-Transferase/GST
Additional Glutathione S-Transferase/GST Products
Product Documents for Glutathione S-Transferase/GST Antibody (PIP-1D7-54.3)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Glutathione S-Transferase/GST Antibody (PIP-1D7-54.3)
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars