Glutathione S-Transferase/GST Antibody

Catalog #: MAB94991 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB94991-100
MAB94991-SP

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Detection of Glutathione S‑Transferase/GST by Western Blot.

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All Glutathione S-Transferase/GST Antibodies

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Glutathione S-Transferase/GST Antibody Summary

Specificity

Detects Glutathione S‑Transferase/GST in direct ELISAs.

Source

Monoclonal Hamster IgG Clone # PIP-1D7-54.3

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

GST-tagged recombinant protein

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5-1 µg/mL

Sf 21 S. frugiperda insect ovarian cell line transfected GST tagged Parkin; E. coli transfected with GST tagged BRDG1-SH2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Glutathione S‑Transferase/GST by Western Blot. Western blot shows lysates ofSf 21 S. frugiperdainsect ovarian cell line mock transfected or transfected GST tagged Parkin. PVDF membrane was probed with 1 µg/mL of Hamster Anti-Glutathione S‑Transferase/GST Monoclonal Antibody (Catalog # MAB94991) followed by HRP-conjugated Anti-Hamster IgG Secondary Antibody. A specific band was detected for Glutathione S‑Transferase/GST tagged Parkin at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot

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Detection of Glutathione S‑Transferase/GST by Western Blot. Western Blot shows lysates of E. coli transfected with GST tagged BRDG1-SH2. PVDF membrane was probed with 0.5 µg/ml of Hamster Anti-Glutathione S‑Transferase/GST Monoclonal Antibody (Catalog # MAB94991) followed by HRP-conjugated Anti-Hamster IgG Secondary Antibody. A specific band was detected for Glutathione S‑Transferase/GST at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.