Recombinant Human GOT2 His Protein

Novus Biologicals | Catalog # NBP2-52146

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

Enzyme Activity, SDS-PAGE
View all GOT2 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 30-430 of Human GOT2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK

Purity

>95%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 60 units/mg, and is defined as the amount of enzyme that convert 1umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GOT2 His Protein

SDS-PAGE: Recombinant Human GOT2 His Protein [NBP2-52146]

SDS-PAGE: Recombinant Human GOT2 His Protein [NBP2-52146]

SDS-Page: Recombinant Human GOT2 Protein [NBP2-52146] - 15% SDS Page (3 ug)

Formulation, Preparation, and Storage

NBP2-52146
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT, 0.15 M NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GOT2

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Alternate Names

aspartate aminotransferase, mitochondrial, EC 2.6.1, EC 2.6.1.1, FABP-1, FABPpm, Fatty acid-binding protein, FLJ40994, Glutamate oxaloacetate transaminase 2, glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2), KAT4, KATIV, kynurenine aminotransferase IV, mAspAT, mitAAT, Plasma membrane-associated fatty acid-binding protein, Transaminase A

Gene Symbol

GOT2

Additional GOT2 Products

Product Documents for Recombinant Human GOT2 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human GOT2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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