H2DCFDA

Tocris Bioscience | Catalog # 5935

Fluorescent ROS indicator; cell permeable
Tocris Bioscience

Key Product Details

Description

Fluorescent ROS indicator; cell permeable
View all Fluorescent Cell Indicators and Sensors »

Product Description

H2DCFDA is a fluorescent ROS indicator; becomes fluorescent when oxidized. Excitation wavelength ~490 nm, emission ~520 nm. Cell permeable.

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Product Specifications for H2DCFDA

Molecular Weight

487.29

Formula

C24H16Cl2O7

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid

CAS Number

4091-99-0

PubChem ID

77718

InChI Key

PXEZTIWVRVSYOK-UHFFFAOYSA-N

SMILES

ClC1=CC(C(C2=CC=CC=C2C(O)=O)C(C=C(Cl)C(OC(C)=O)=C3)=C3O4)=C4C=C1OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 48.73 100
Ethanol 4.87 10

Preparing Stock Solutions for H2DCFDA

The following data is based on the product molecular weight 487.29.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.05 mL 10.26 mL 20.52 mL
5 mM 0.41 mL 2.05 mL 4.10 mL
10 mM 0.21 mL 1.03 mL 2.05 mL
50 mM 0.04 mL 0.21 mL 0.41 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 13 publications citing the usage of this product.

Product Documents for H2DCFDA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for H2DCFDA

For research use only

Customer Reviews for H2DCFDA (3)

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  • H2DCFDA
    Name: Anonymous
    Species: Human
    Assay Type: In Vivo
    Cell Line/Tissue: HepG2
    Verified Customer | Posted 09/06/2025
    I used H2DCFDA to measure the overproduction of ROS by HepG2 cells under hypoxic and hyperglycemic conditions. Cells were treated with 10 μM of H2DCFDA for 30 min at 37 0C and imaged under a fluorescent microscope.
    H2DCFDA 5935
  • H2DCFDA detects intracellular ROS levels in 3D Glioblastoma cell lines
    Name: sara Azeem
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: G7
    Verified Customer | Posted 01/08/2020
    Try different dilutions to get the one best suited to you. We treated the cells for 30 minutes and that was enough to get robust results
    Glioblatsoma cell line G7 was cultured in 3D sphere model and the levels of intracellular ROS were measured.
    H2DCFDA 5935
  • H2DCFDA detects intracellular ROS levels in 15d-PGJ2 treated cardiomyocytes
    Name: Chintan Koyani
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: HL-1 cardiomyocytes
    Verified Customer | Posted 10/13/2018
    HL-1 cardiomyocytes were stimulated with 15d-PGJ2 up to 60 min to follow intracellular ROS levels using H2DCFDA.
    H2DCFDA 5935

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