HeLa Whole Cell Lysate

Novus Biologicals | Catalog # NB800-PC1

Novus Biologicals
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Key Product Details

Species

Human

Applications

Western Blot

Product Specifications

Application Notes

This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.

Type

Cell

Tissue

Reproductive

Subcellular Fraction

Whole

Scientific Data Images for HeLa Whole Cell Lysate

Western Blot: HeLa Whole Cell Lysate [NB800-PC1]

Western Blot: HeLa Whole Cell Lysate [NB800-PC1]

Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.
Western Blot: HeLa Whole Cell Lysate [NB800-PC1]

Western Blot: HeLa Whole Cell Lysate [NB800-PC1]

Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Ponceau S staining of HeLa whole cell lysate using NB800-PC1.
Western Blot: HeLa Whole Cell Lysate [NB800-PC1]

Western Blot: HeLa Whole Cell Lysate [NB800-PC1]

Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Analysis of STIM-1 antibody (NB110-60547) using HeLa whole cell lysate [NB800-PC1].
Western Blot: HeLa Whole Cell Lysate [NB800-PC1]

Western Blot: HeLa Whole Cell Lysate [NB800-PC1]

Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of 30ug of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal Survivin antibody (NB 500-201) at 1ug/ml concentration. Detection was performed using ECL based method with 1 minute exposure time.
Western Blot: HeLa Whole Cell Lysate [NB800-PC1]

Western Blot: HeLa Whole Cell Lysate [NB800-PC1]

Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal NBS1 antibody (NB100-143) with detection employing ECL method.

Formulation, Preparation, and Storage

Formulation

Standard RIPA buffer including protease inhibitors and 0.1% SDS diluted in 1 X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. Denaturing agents have not been added.

Preservative

No Preservative

Concentration

2.5 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HeLa

HeLa whole cell lysate was lysed in modified RIPA buffer (150 mM sodium chloride, 25 mM Tris-HCl, pH 7.6, 1% Triton X-100, 1% sodium deoxycholic acid, 1% sodium dodecyl sulfate, 1 mM phenyl methyl sulfonyl fluoride, 1 ug/ml of aprotinin, 1 ug/ml of leupeptin, 1ug/ml of pepstatin). Protein concentration was determined with Bio-Rad protein assay.

Additional HeLa Products

Product Documents for HeLa Whole Cell Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HeLa Whole Cell Lysate

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Citations for HeLa Whole Cell Lysate

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