HEPES

Tocris Bioscience | Catalog # 3173

Multipurpose HEPES buffer; used in cell culture
Tocris Bioscience

Key Product Details

Description

Multipurpose HEPES buffer; used in cell culture
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Product Description

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media. HEPES can be used in a small molecule cocktail to generate 3D Culture of Lung Alveolar Cells (see our protocol below).

For more information about how HEPES may be used, see our protocol: 3D Culture of Lung Alveolar Cells

Product Specifications for HEPES

Molecular Weight

238.30

Formula

C8H18N2O4S

Storage

Store at RT

Purity

≥99.5%

Chemical Name

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

CAS Number

7365-45-9

PubChem ID

23830

InChI Key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

SMILES

OCCN1CCN(CCS(=O)(O)=O)CC1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 238.3 1000

Preparing Stock Solutions for HEPES

The following data is based on the product molecular weight 238.30.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
10 mM 0.42 mL 2.10 mL 4.20 mL
50 mM 0.08 mL 0.42 mL 0.84 mL
100 mM 0.04 mL 0.21 mL 0.42 mL
500 mM 0.01 mL 0.04 mL 0.08 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 567 publications citing the usage of this product.

  • Medzon and Gedies Substitution of 4-(2-hydroxyethyl)-1-piperazineethane sulfonic acid (HEPES) for bicarbonate in protein-free animal cell culture medium: application to vaccinia virus quantitation and fluorogenic acetylesterase assay in living LM cells. Canadian J.Microbiol.
  • Good Hydrogen ion buffers for biological research. Biochemistry 1966 PMID: 5942950

Product Documents for HEPES

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for HEPES

For research use only

Customer Reviews for HEPES (5)

4.6 out of 5
5 Customer Ratings
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4 Stars
40%
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1 Stars
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  • HEPES (10 mM) maintains pH in the experimental buffer for Ca2+ imaging.
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Verified Customer | Posted 07/02/2019
    HEPES (10 mM) is used to maintain pH 7.4 in the experimental buffer for Ca2+ imaging, consisting of 137 mM NaCl, 5.4 mM KCl, 1 mM CaCl2, 1 mM MgCl2 and 10 mM glucose.
  • Primary cell culture
    Name: Oonagh Paerewijck
    Assay Type: In Vitro
    Cell Line/Tissue: miccl2
    Verified Customer | Posted 09/04/2018
    Used at 20 mmol/L in DMEM buffer for primary cell culture
    HEPES 3173
  • Good buffer
    Name: Anonymous
    Species: Mouse
    Assay Type: Ex Vivo
    Verified Customer | Posted 08/05/2018
    Used to buffer Krebs buffer for pressure myography. Good performance, maintained pH without any problem. I would use it again.
  • Media ingredient
    Name: Danlee Enzler
    Verified Customer | Posted 05/01/2018
    Additive in cell culture media
  • HEPES to buffer pH
    Name: Anonymous
    Species: Mouse
    Assay Type: Ex Vivo
    Cell Line/Tissue: Brain slice
    Verified Customer | Posted 01/31/2018
    HEPES used in solutions, good pH buffer.

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