HGF activator (HGFA) is a serine protease that cleaves the single-chain HGF precursor, generating the active heterodimer. HGFA is secreted by the liver and normally circulates in the blood as an inactive zymogen, which can be activated by thrombin. The active protease is regulated by HGFA inhibitors.
HGF Activator Antibody (196817) [Dylight 488]
Novus Biologicals | Catalog # FAB1583K
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
DyLight 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 196817
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pro HGF Activator
Gln36-Ser655
Accession # Q04756
Gln36-Ser655
Accession # Q04756
Specificity
Detects human Pro HGF Activator in direct ELISAs and Western blots. It recognizes only the propeptide region of HGFA precursor, not active HGFA. In Western blots, 100% cross-reactivity with recombinant mouse Pro HGFA is observed and less than 5% cross-reactivity with recombinant human (rh) Factor X and rhThrombin is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for HGF Activator Antibody (196817) [Dylight 488]
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: HGF Activator
Long Name
Hepatocyte Growth Factor Activator
Alternate Names
HGFA, Hgfac
Gene Symbol
HGFAC
Additional HGF Activator Products
Product Documents for HGF Activator Antibody (196817) [Dylight 488]
Product Specific Notices for HGF Activator Antibody (196817) [Dylight 488]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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