HSD17B3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-04666

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSD17B3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human HSD17B3 (NP_000188.1). MGDVLEQFFILTGLLVCLACLAKCVRFSRCVLLNYWKVLPKSFLRSMGQWAVITGAGDGIGKAYSFELAKRGLNVVLISRTLEKLEAIATEIERTTGRSVKIIQADFTKDDIYEHIKEKLAGLEIGILVNNVGMLPNLLPSHFLNAPDEIQSLIHCNITSVVKMTQLILKHMESRQKGLILNISSGIALFPWPLYSMYSASKAFVCAFSKALQEEYKAKEVIIQVLTPYAVSTAMTKYLNTNVITKTADEFVKESLNYVTIGGETCGCLAHEILAGFLSLIPAWAFYSGAFQRLLLTHYVAYLKLNTKVR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSD17B3 Antibody - BSA Free

HSD17B3 Antibody - BSA Free

Western Blot: HSD17B3 Antibody - BSA Free [NBP3-04666] -

Western Blot: HSD17B3 Antibody - BSA Free [NBP3-04666] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with HSD17B3 using HSD17B3 Rabbit pAb (A7687) at 1:2500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Applications for HSD17B3 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSD17B3

HSD17B3 is an isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.

Alternate Names

EC 1.1.1.64, EDH17B317-beta-HSD3, hydroxysteroid (17-beta) dehydrogenase 3,17-beta-hydroxysteroid dehydrogenase type 3, SDR12C2, short chain dehydrogenase/reductase family 12C, member 2, Testicular 17-beta-hydroxysteroid dehydrogenase, testosterone 17-beta-dehydrogenase 3,17-beta-HSD 3

Gene Symbol

HSD17B3

Additional HSD17B3 Products

Product Documents for HSD17B3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HSD17B3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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