HSP70/DnaK Antibody (8E2/2) - BSA Free

Novus Biologicals | Catalog # NBP1-97490

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, E. coli

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8E2/2

Format

BSA Free
View all HSP70/DnaK Primary Antibodies »

Product Specifications

Immunogen

E. coli DnaK.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33918708)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP70/DnaK Antibody (8E2/2) - BSA Free

Western Blot: HSP70/DnaK Antibody (8E2/2) [NBP1-97490]

Western Blot: HSP70/DnaK Antibody (8E2/2) [NBP1-97490]

Western Blot: HSP70/DnaK Antibody (8E2/2) [NBP1-97490] - HSP70/HSPA1A Antibody (8E2/2) [NBP1-97490] - Analysis: Lane 1: MW Marker, Lane 2: DnaK active recombinant protein, Lane 3: E. coli cell lysate.

Applications for HSP70/DnaK Antibody (8E2/2) - BSA Free

Application
Recommended Usage

Simple Western

1:120

Western Blot

1:10000
Application Notes
Use in Western Blot reported in scientific literature (PMID:33918708)

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP70/DnaK

DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.

Alternate Names

chaperone protein DnaK, ECK0014, groPAB, groPC, groPF, grpC, grpF, SEG

Gene Symbol

dnaK

UniProt

P08107, P0A6Y8 (E. coli)

Additional HSP70/DnaK Products

Product Documents for HSP70/DnaK Antibody (8E2/2) - BSA Free

Certificate of Analysis

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Product Specific Notices for HSP70/DnaK Antibody (8E2/2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HSP70/DnaK Antibody (8E2/2) - BSA Free

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