PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.
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HT PARP in vivo Pharmacodynamic ELISA Kit II
R&D Systems | Catalog # 4520C-096-K
For the quantitation of PAR in tissues and cultured cells.
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Key Product Details
Features
Monitors in vivo PARP activity within cells based on levels of PAR.
Key Benefits
- Pre-coated 96 well capture antibody plates
- High signal to noise ratio
- Detection sensitivity of 15.6 pg/mL of PAR
- Broad linear dynamic range to 1,000 pg/mL
- Reduced inter-assay variability
- Validated assay that measures drug action on PARP in both in vivo and in vitro settings
- 96 test size
Species
Multi-Species
Product Summary for HT PARP in vivo Pharmacodynamic ELISA Kit II
A polyclonal antibody specific for PAR has been pre-coated onto a microplate to capture cellular PAR and PAR attached to proteins. Incubation with a PAR Monoclonal Detection Antibody, followed by addition of a chemiluminescent HRP substrate yields relative light units (RLU) that directly correlate with the amount of cellular PAR.
Product Specifications
- Quantitation of PAR in tissue lysates and culture cells.
- Monitoring the efficacy of PARP inhibitors on PAR formation in vivo.
- Verifying observations of enhanced cancer cell cytotoxicity arising from PARP inhibitor/anticancer drug combination therapy.
- Facilitating development of PARP and PARG targeted therapeutics.
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Scientific Data Images for HT PARP in vivo Pharmacodynamic ELISA Kit II
HT PARP in vivo Pharmacodynamic ELISA Kit II Curve
Kit Contents for HT PARP in vivo Pharmacodynamic ELISA Kit II
- PARP Microplate coated with PAR polyclonal antibody
- Calibrator Diluent RD5-20////PARP Cell Lysis Reagent
- 100X Magnesium cation
- Assay Diluent RD1-117
- PAR Monoclonal Detection Antibody
- PeroxyGlow A and B
- Wash Buffer Concentrate
- PAR Standard
- PAR Control, Low and High
- DNase I, 2 Units/uL
- 20% SDS
- Plate Sealers
Formulation, Preparation, and Storage
Shipping
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at 2 to 8 °C. Store the DNAse at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: PARP
Long Name
Poly [ADP-ribose] Polymerase
Alternate Names
ADPRT, PARP1, PPOL
Additional PARP Products
Product Documents for HT PARP in vivo Pharmacodynamic ELISA Kit II
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
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