Human ABCB5 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF5799T Datasheet / COA / SDS

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AF5799T-100UG

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All ABCB5 Antibodies

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Human ABCB5 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ABCB5 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ABCB5
Ile141-Val247
Accession # Q2M3G0

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ABCB5

ABCB5 (ATP-binding cassette, subfamily B [DR/TAP] member 5) is a 90 kDa (predicted MW) member of the human P-glycoprotein family of molecules. It is expressed by CD133+ pluripotent pigment stem cells, where it serves as a marker for cells that show a high incidence of polyploidy. Human ABCB5 is 812 amino acids (aa) in length. It is possibly a five transmembrane protein with a 247 aa N-terminal extracellular domain (ECD) (aa 1-247) and a 283 aa C-terminal cytoplasmic region (aa 530-812). The ECD contains one ABC transporter (aa 2-177), while the cytoplasmic region contains a second ABC transporter (aa 570-808). There are two potential splice forms. One shows a seven aa substitution for aa 125-812, while another possesses an alternative start site 445 aa upstream of the standard site. Over aa 141-247, human ABCB5 shares 64% aa identity with mouse ABCB5.

Long Name

ATP-binding Cassette, Sub-family B (MDR/TAP), Member 5

Entrez Gene IDs

340273 (Human); 77706 (Mouse); 314537 (Rat)

Alternate Names

ABCB5 P-gp; ABCB5; ABCB5alpha; ABCB5beta; ATP-binding cassette protein; ATP-binding cassette sub-family B member 5; ATP-binding cassette, sub-family B (MDR/TAP), member 5; EC 3.6.3; EC 3.6.3.44; EST422562; P-glycoprotein ABCB5