Human Adenosylhomocysteinase/AHCY Alexa Fluor™ Plus 594‑conjugated Antibody

R&D Systems | Catalog # AF6466AFP594

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Polyclonal Sheep IgG
View all Adenosylhomocysteinase/AHCY Primary Antibodies »

Product Specifications

Specificity

Detects human Adenosylhomocysteinase/AHCY in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Adenosylhomocysteinase/AHCY

Human S‑Adenosylhomocysteinase (AHCY) is a cytoplasmic tetramer with a tightly bound NAD co‑factor for each subunit (1, 2). It is the only known enzyme to catalyze the breakdown of S‑adenosylhomocysteine (AdoHcy) to homocysteine and adenosine. AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCY’s activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease (3). It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S‑adonosyl-L-methionine methyltransferases (1). A mutation in the human AHCY results in AHCY deficiency with increase of plasma creatine kinase, methionine, S‑adenosylmethionine and AdoHcy, delay of myelination, myopathy and psychomotor retardation (4, 5).

References

  1. Turner, M. A. et al. (2000) Cell Biochem. Biophys. 33:101.
  2. Takata, Y. et al. (2002) J. Biol. Chem. 277:22670.
  3. Gellekink, H. et al. (2004) Eur. J. Hum. Genet. 12:942.
  4. Baric, I. et al. (2004) Proc. Natl. Acad. Sci. USA. 101:4234.
  5. Fumic, K. et al. (2007) Eur. J.Hum. Genet. 15:347. 

Alternate Names

AdoHcyase, AHCY, SAHH

Entrez Gene IDs

191 (Human)

Gene Symbol

AHCY

UniProt

P23526 (Human)

Additional Adenosylhomocysteinase/AHCY Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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