Human AIM2 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB9965V Datasheet / COA / SDS

Recombinant Monoclonal Antibody

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FAB9965V-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human AIM2 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human AIM2 in direct ELISAs.

Source

Monoclonal Rabbit IgG Clone # 2307A

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human AIM2
Lys79-Thr180
Accession # O14862

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AIM2

Human Interferon-inducible protein AIM2 (also known as absent in melanoma 2 or simply AIM2), encoded by the AIM2 gene, is a 343 amino acids (aa), 39 KDa protein with a N-terminal DAPIN (or pyrin) domain (amino acids 1-87) and a C-terminal HIN-200 domain (amino acids 138-337), which is known to have two oligonucleotide-binding folds. Recent research has shown that AIM2 is part of the inflammasome and is involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating the inflammasome in macrophages. Upon binding to DNA, Aim-2 undergoes oligomerization and associates with PYCARD initiating the recruitment of caspase-1 precursors and processing of IL-1b and IL-18. Over aa 71-180, human Aim2 shares 90% aa sequence identity with macaque Aim2.

Long Name

Absent In Melanoma 2

Entrez Gene IDs

9447 (Human); 383619 (Mouse); 304987 (Rat)

Alternate Names

absent in melanoma 2PYHIN4; AIM2; Gm1313; Ifi210; interferon-inducible protein AIM2; PYHIN4