Human Ajuba Alexa Fluor® 532-conjugated Antibody

Catalog #: AF5594X Datasheet / COA / SDS

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AF5594X-100UG

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All Ajuba Antibodies

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Human Ajuba Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human Ajuba in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Ajuba
Met1-Arg121
Accession # Q96IF1

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Ajuba

Ajuba (an Urdu word meaning "curiosity") is a 55‑60 kDa member of the Zyxin/Ajuba family of proteins. Ajuba is found in keratinocytes, astrocytes and neurons, and exhibits multiple functions. It shuttles between the nucleus and cytosol, and participates in alpha -catenin:F-actin binding, beta -catenin binding and phosphorylation, p62 aPKC scaffold protein binding and NF-kappa B activation, and Aurora-A binding and phosphorylation. Human Ajuba is 538 amino acids (aa) in length. It contains a Pro-rich preLIM region that binds actin and Grb2 (aa 1‑335), an NES (aa 280‑287), and three LIM domains that bind multiple proteins (aa 336‑397, 401‑461 and 462‑530).

Entrez Gene IDs

84962 (Human); 16475 (Mouse); 85265 (Rat)

Alternate Names

Ajuba; JUB; jub, ajuba homolog (Xenopus laevis); MGC15563; protein ajuba