Human Akt Pan Specific Antibody

R&D Systems | Catalog # AF2055

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Akt synthetic peptide
IRCLQWTTVIERTFHV
Accession # P31749

Specificity

Detects human Akt1, Akt2, and Akt3 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Akt Pan Specific Antibody

Detection of Human Akt antibody by Western Blot.

Detection of Human Akt by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, MCF-7 human breast cancer cell line, and KG-1 human myeloid leukemia cell lines. PVDF membrane was probed with 0.1 µg/mL of Human Akt Pan Specific, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Akt Pan Specific, at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Akt by Simple WesternTM.

Simple Western lane view shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Akt at approximately 60 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human Akt Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2055). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Akt Pan Specific Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: HDLM‑2 human Hodgkin's lymphoma cell line

Western Blot

0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, MCF-7 human breast cancer cell line, and KG-1 human myeloid leukemia cell lines

Reviewed Applications

Read 1 review rated 4 using AF2055 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Akt

Rabbit antibodies were raised against a synthetic peptide corresponding to amino acids 75‑90 of human v-akt murine thymoma viral oncogene homolog 1 (Akt1), also known as protein kinase B alpha (PKB alpha ) and RAC-alpha serine/threonine kinase (RAC-PK-alpha ). In addition, this peptide sequence is identical to amino acids 75‑90 of human Akt2 (PKB beta, RAC-PK-beta ), and amino acids 74‑89 of human Akt3 (PKB gamma, RAC-PK-gamma ).

Long Name

v-Akt Murine Thymoma Viral Oncogene Homolog

Alternate Names

PKB, RAC

UniProt

P31749

Additional Akt Products

Product Documents for Human Akt Pan Specific Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Akt Pan Specific Antibody

For research use only

Citations for Human Akt Pan Specific Antibody

Customer Reviews for Human Akt Pan Specific Antibody (1)

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  • Human Akt Pan Specific Antibody
    Name: Maria Russo
    Application: Western Blot
    Sample Tested: Human Chronic Lymphocytic Cell Line
    Species: Human
    Verified Customer | Posted 04/28/2017
    30 microg cell homogenate/line Gel SDS/Page 4-12% BisTris Novex Invitrogen. Western Blot on PVDF membrane. Blocking Reagent: 5% non fat dry milk/T-TBS Anti AKT1-2-3 (R& D System cat. N. AF2055) 1:1250 in 3% BSA/PBS o.n. 4°C Anti pAKTser473 Cell Signalling 1:1250 in 3% BSA/PBS o.n. 4°C. Anti horsadish peroxidase linked II antibody against Rabbit 1:20.000 (GE Healthcare) in T-TBS 90 min r.t. ECL Prime Amersham (GE Healhtcare).
    Human Akt Pan Specific Antibody AF2055

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