Human Angiostatin Antibody

Catalog #: AF226X Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF226X-100UG

Save 15% on Select RUO Reagents. See Details.

All Angiostatin Antibodies

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Angiostatin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Angiostatin in direct ELISAs and Western blots. This antibody will also recognize human plasminogen.

Source

Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Angiostatin Kringle 1-3
Val98-Ser358 with Cys188Ser & Cys316Ser substitutions
Accession # P00747

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Angiostatin

Angiostatin is a 38-45 kDa proteolytic fragment of plasminogen, a 92-100 kDa glycosylated blood zymogen that serves as the precursor for plasmin. Mature human plasminogen is 713 amino acids (aa) in length (aa 98-810). It contains five N-terminal kringle domains (aa 103-561) plus one peptidase S1 domain (aa 581-808). Upon cleavage by tPA between Arg580 and Val581, plasminogen is converted into the disulfide-linked two-subunit enzyme plasmin that dissolves fibrin clots. Plasminogen is also cleaved by MMPs which generate multiple fragments. Angiostatin has documented antiangiogenic and antitumor activity, likely mediated by a downregulation of Bcl2, and an upregulation of thrombospondin-1. Over aa 98-358, human angiostatin shares 81% aa identity with mouse angiostatin.

Entrez Gene IDs

5340 (Human); 18815 (Mouse)

Alternate Names

Angiostatin; HAE4