Human AP-2 epsilon Alexa Fluor® 594-conjugated Antibody

Catalog #: AF5060T Datasheet / COA / SDS

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AF5060T-100UG

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All AP-2 epsilon Antibodies

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Human AP-2 epsilon Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human AP-2 epsilon in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) AP‑2 alpha, rhAP‑2 beta, rhAP‑2δ, and rhAP‑2 gamma is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human AP-2Îµ
Ala123-Val217
Accession # Q6VUC0

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AP-2 epsilon

AP-2 epsilon (Activating protein 2 epsilon) is a 48 kDa member of the AP-2 transcription factor family. It is found in embryonic olfactory bulb mitral cells and adult keratinocytes and chondrocytes. In the nucleus, it presumably forms homodimers and heterodimers with other AP‑2 family members. Human AP‑2 epsilon is 442 amino acids in length. It contains a Gln/Pro-rich transactivation domain (aa 22‑122), a DNA binding domain (aa 216‑286) and an AP‑2, helix‑span‑helix dimerization region (aa 287‑417). There is one potential alternate start site at Met09. Over aa 123‑217, human AP‑2 epsilon shares 86% aa identity with mouse AP‑2 epsilon.

Long Name

Activating Enhancer-binding Protein epsilon

Entrez Gene IDs

339488 (Human)

Alternate Names

Activating enhancer-binding protein 2-epsilon; adaptor-related protein complex 2, epsilon subunit; AP2 epsilon; AP-2 epsilon; AP2E; AP2-epsilon; MGC49007; TFAP2E; transcription factor AP-2 epsilon (activating enhancer binding protein 2epsilon); transcription factor AP-2-epsilon