Human APAF-1 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB868V Datasheet / COA / SDS

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FAB868V-100UG

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All APAF-1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human APAF-1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human APAF-1 in Western blots.

Source

Monoclonal Mouse IgG2b Clone # 94408

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human APAF-1
Leu10-Asp214
Accession # AAC51678

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: APAF-1

Apoptosis protease-activating factor 1 (APAF‑1) is a ubiquitous 135 kDa protein component of the cytoplasmic apoptosome complex. APAF‑1 plays an important role in the mitochondrial apoptotic pathway by triggering the Cytochrome c-dependent activation of Caspase-9. APAF‑1 participates in tissue morphogenesis during development, and loss of APAF‑1 expression is associated with tumor progression in a variety of cancers. Some alternate splice forms of APAF‑1 are deficient in their ability to promote apoptosis.

Long Name

Apoptotic Peptidase Activating Factor 1

Entrez Gene IDs

317 (Human)

Alternate Names

APAF1; APAF-1; APAF-1apoptotic protease activating factor 1; apoptotic peptidase activating factor 1; apoptotic peptidase activating factor; apoptotic protease activating factor; apoptotic protease-activating factor 1; CED4; DKFZp781B1145; KIAA0413