Human Apolipoprotein B100/ApoB100 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF3260V Datasheet / COA / SDS

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AF3260V-100UG

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All Apolipoprotein B100/ApoB100 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Apolipoprotein B100/ApoB100 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ApoB100 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ApoB100
Ile3374-Val3600
Accession # AAP72970

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Apolipoprotein B100/ApoB100

ApoB100 is a major serum Apolipoprotein synthesized by the liver. It is an important constituent of LDL, VLDL and IDL particles. It interacts with the LDL receptor and is involved in the delivery of cholesterol to tissues. Within the peptide sequence of the immunogen, human and mouse ApoB100 share 74% amino acid sequence identity. The sequence within the immunogen is absent in ApoB48, an alternative splice isoform that is exclusively synthesized by the gut. ApoB48 contains the amino terminal 47% of ApoB100 and lacks the LDL receptor region.

Entrez Gene IDs

338 (Human); 238055 (Mouse); 54225 (Rat)

Alternate Names

apoB-100;apoB-48;Apolipoprotein B-100;FCHL2;FLDB;LDLCQ4; Apolipoprotein B100