Human Apolipoprotein M/ApoM Alexa Fluor® 700-conjugated Antibody

Catalog #: AF4550N Datasheet / COA / SDS

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AF4550N-100UG

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Human Apolipoprotein M/ApoM Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human ApoM in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ApoM
Cys23-Asn188
Accession # O95445

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Apolipoprotein M/ApoM

Apolipoprotein M (ApoM; also known as Protein G3a) is a 25 kDa, secreted member of the lipocalin family of proteins. It is produced by hepatocytes and renal tubular epithelium, and represents a minor component of HDL particles. Human ApoM is 188 amino acids (aa) in length. It contains an uncleaved, hydrophobic N-terminal segment and one utilized N-linked glycosylation site (mouse ApoM shows no glycosylation). At least two potential isoforms exist. One shows an alternate start site at Met73, while a second shows the same start site plus a 21 aa substitution for aa 181‑188. Human ApoM shares 91% and 80% aa identity with pig and mouse ApoM, respectively.

Entrez Gene IDs

55937 (Human); 55938 (Mouse); 55939 (Rat)

Alternate Names

Apolipoprotein M; APOM; apo-M; G3A; HSPC336; MGC22400; NG20; NG20-like protein; Protein G3a