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Human ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody

Catalog #: MAB4494
3 Citations Datasheet / COA / SDS
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MAB4494
MAB4494-SP

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Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody Summary

Species Reactivity
Human
Specificity
Detects human ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse ASAHL is observed and no cross-reactivity with recombinant human ASAH2 or recombinant mouse ASAH2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 511702
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ASAHL/N‑acylethanolamine-hydrolyzing Acid A isoform 1
Ser29-Lys359 (Val151Ile)
Accession # Q02083
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4494‑AH)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4494-AH), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase

The human NAAA gene encodes N-acylethanolamine-hydrolyzing acid amidase, also known as ASAH-like protein (ASAHL), a fatty acid amidase with maximal activity at acidic pH (1). ASAHL hydrolyzes a number of N-acyl ethanolamines, including N-myristoyl-, N-stearoyl, N-oleoyl, and N-arachidonoyl, but is most active against N‑palmitoylethanolamine (2). ASAHL is a member of the cholylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (3). ASAHL is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (3). ASAHL can be distinguished from anandamide amidohydrolase by its lack of inhibition by methyl arachidonyl fluorophosphonate (2).

References
  1. Hong, S.B. et al. (1999) Genomics 62:232.
  2. Ueda, N. et al. (2001) J. Biol. Chem. 276:35552.
  3. Tsuboi, K. et al. (2005) J. Biol. Chem. 280:11082.
Entrez Gene IDs
27163 (Human); 67111 (Mouse); 497009 (Rat)
Alternate Names
Acid ceramidase-like protein; ASAHL; ASAH-like protein; EC 3.5.1.-; NAAA; N-acylethanolamine acid amidase; Nacylethanolaminehydrolyzing Acid Amidase; N-acylethanolamine-hydrolyzing acid amidase; N-acylsphingosine amidohydrolase (acid ceramidase)-like; N-acylsphingosine amidohydrolase-like; PLT

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Citations for Human ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Molecular Insights into the Nociceptive Modulation by Palmitoylethanolamide and Equisetum arvense Extract: An In Vitro Study Across the Blood-Brain Barrier
    Authors: Mulè, S;Galla, R;Ferrari, S;Invernizzi, M;Uberti, F;
    Nutrients
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Antinociceptive effects of the N-acylethanolamine acid amidase inhibitor ARN077 in rodent pain models
    Authors: Oscar Sasso, Guillermo Moreno-Sanz, Cataldo Martucci, Natalia Realini, Mauro Dionisi, Luisa Mengatto et al.
    Pain
  3. Antinociceptive effects of the N-acylethanolamine acid amidase inhibitor ARN077 in rodent pain models
    Authors: Oscar Sasso, Guillermo Moreno-Sanz, Cataldo Martucci, Natalia Realini, Mauro Dionisi, Luisa Mengatto et al.
    Pain
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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