Human Autoimmune Regulator/AIRE Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Autoimmune Regulator/AIRE

AIRE (Autoimmune Regulator; also APECED) is a 58‑60 kDa member of the PHD Zn-finger family of transcription factors. It appears to have restricted expression, being identified in thymic medullary epithelium, dendritic cells and neutrophils. AIRE regulates the expression of self-antigens in thymus. In particular, genes coding for these antigens are generally inactive, and show minimal H3K4me3 and AcH3 reactivity in their promoters. Human AIRE is 545 amino acids (aa) in length. It contains multiple domains, including an HSR domain that mediates homodimerization (aa 1‑105), an NLS (aa 131‑133), a SAND domain (aa 181‑280), and two Zn-finger PHD domains (aa 296‑343 and 434‑475) that bind to nonmethylated histone H3. There are isoforms that show a Trp substitution for aa 437‑467, a 95 aa substitution for aa 1‑292, and a 75 aa substitution for aa 377‑545. Over aa 476‑545, human AIRE shares 71% aa identity with mouse AIRE.

Product Datasheets

Related Research Areas

• Transcriptional Regulators in Allergy and Asthma