Human BACE-2 Ectodomain Antibody

R&D Systems | Catalog # AF4097

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all BACE-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BACE-2 Ectodomain
Gly63-Pro466
Accession # Q9Y5Z0

Specificity

Detects human and mouse BACE‑2 Ectodomain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) BACE‑1, rhCathepsin D, and rhCathepsin E is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human BACE-2 Ectodomain Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human BACE‑2, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample:

Recombinant Human BACE‑2

Recombinant Mouse BACE-2 (Catalog # 2977-BA)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BACE-2

BACE-2 ( beta -site APP cleaving enzyme 2; also ASP-1 and Memapsin-1) is a 73‑75 kDa member of the peptidase A1 family. It is expressed both inside and outside the CNS and is classified as a Golgi aspartyl protease. Its status as a beta -secretase is unclear. If it has physiological activity, it may be associated with glia. Mature BACE-2 is a 456 amino acid (aa) type I transmembrane glycoprotein. It contains a 411 aa extracellular domain (aa 63‑473) that has two catalytic residues (Asp93 and Asp289). Two potential splice forms exist; one shows a deletion of aa’s 329‑378, while a second shows an 18 aa substitution for aa’s 380‑518. Over aa 63‑466, human BACE-2 shares 92% aa sequence identity with mouse BACE‑2.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 2

Alternate Names

BACE2

Entrez Gene IDs

25825 (Human); 56175 (Mouse); 288227 (Rat)

Gene Symbol

BACE2

UniProt

Q9Y5Z0

Additional BACE-2 Products

Product Documents for Human BACE-2 Ectodomain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human BACE-2 Ectodomain Antibody

For research use only

Related Research Areas

Aspartic Proteases and Regulators

Citations for Human BACE-2 Ectodomain Antibody

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