BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.
Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA
R&D Systems | Catalog # QK0124
Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Summary for Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA
Product Specifications
Measurement
Detection Method
Conjugate
Species
Specificity
Cross-reactivity
Interference
精度
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 81.2 | 605 | 96.2 | 558 |
| Standard Deviation | 2.28 | 21.9 | 10.5 | 34.3 |
| CV% | 2.8 | 3.6 | 10.9 | 6.2 |
Recovery for Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA
The recovery of BAFF is spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 97 | 85-105 |
| EDTA Plasma (n=2) | 101 | 85-110 |
| Heparin Plasma (n=2) | 99 | 81-108 |
| Lysis Buffer (n=1) | 125 | 117-133 |
| Serum (n=2) | 95 | 78-104 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of BAFF were diluted with Calibrator Diluent RD5-20 (diluted 1:2) to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA
Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: BAFF/BLyS/TNFSF13B
Long Name
Alternate Names
Gene Symbol
Additional BAFF/BLyS/TNFSF13B Products
Product Documents for Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA
For research use only
Customer Reviews for Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA
There are currently no reviews for this product. Be the first to review Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA and earn rewards!
Have you used Human BAFF/BlyS/TNFSF13B Quantikine QuicKit ELISA?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Associated Pathways
