Human BAI1 Antibody

R&D Systems | Catalog # AF4969

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Neutralization

Cited:

Neutralization, Flow Cytometry, Bioassay, Western Blots

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all BAI1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human BAI1
Ala31-Thr879
Accession # O14514

Specificity

Detects human BAI1 in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human BAI3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human BAI1 Antibody

Detection of Mouse BAI1 by Immunocytochemistry/Immunofluorescence

Detection of Mouse BAI1 by Immunocytochemistry/Immunofluorescence

Bai1Tg reduces accumulation of apoptotic corpses in Mertk−/− mice post-torsion. (A) Sertoli cell expression of Bai1, BAI1 signaling pathway genes, and Mertk were analyzed by quantitative RT-PCR. Sertoli cells were isolated from Mertk+/+ (n = 4) and Mertk−/− (n = 2) mice and were cultured for 3 days to expand them prior to RNA isolation. Error bars are standard error of mean (SEM). (B) Representative images of isolated Sertoli cells from Mertk−/− and Mertk−/−Bai1Tg mice were stained for BAI1 to confirm surface expression of the Bai1Tg. (C) Mice (8–12 weeks-old) underwent testicular torsion surgery to induce ischemic injury. Testicular cross sections from sham and torsion testes were stained for cleaved caspase 3 (CC3) (black arrowheads). Images are of representative tubule cross sections from matched sham and torsion testes. (D) The number of CC3 positive cells per tubule cross section was determined by analyzing the entire testicular cross section. Each mouse is represented by individual data points within the bars. Mertk+/+ (n = 5) Mertk−/− (n = 8) Mertk−/−Bai1Tg (n = 9). Error bars represent SEM. Statistical analysis was performed with a Wilcoxon rank-sum test. *p < 0.05, ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-15191-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human BAI1 Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize the the enhancemment of adhesion of BCE C/D1b bovine cornea derived endothelial cells induced by BAI-1. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 0.5 µg/mL Recombinant Human BAI-1.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BAI1

Brain Angiogenesis Inhibitor 1 (BAI1) is a 170 kDa 7-transmembrane domain G protein-coupled receptor (GPCR) that has a large N-terminal extracellular region with an RGD motif, five thrombospondin type I repeats, and a juxtamembrane GPS (GPCR proteolytic cleavage site) (1). Within the extracellular domain (ECD) up to the GPS (amino acids 31‑879), mature human BAI1 shares 94% amino acid sequence identity with mouse and rat BAI1. BAI1 is preferentially expressed on brain neurons but also is found on astrocytes and macrophages and in the pancreas, stomach, and colon (1‑8). BAI1 can be cleaved within the GPS to release a 120 kDa fragment termed Vasculostatin which corresponds to nearly the entire N-terminal ECD (9). Generation of additional soluble fragments suggests the cleavage of BAI1 at multiple sites (9, 10). BAI1 fragments interact with Integrin alpha V beta 5 or CD36 on microvascular endothelial cells to inhibit cell proliferation and migration (10, 11). Overexpression of BAI1 in glioblastoma or pancreatic adenocarcinoma cells inhibits their tumorigenicity and the development of tumor-associated neovascularization (6, 12). Fragments of the ECD, including Vasculostatin, also suppress in vivo angiogenesis and tumor growth (1, 9, 11). BAI1 is down‑regulated in glioblastoma, carcinomas of the pancreas, colon, and stomach and also in experimental ischemia (2, 4, 6‑8). Its expression is inversely correlated with tumor vascularity in colorectal and pulmonary carcinomas (8, 13). On macrophages and astrocytes, BAI1 mediates the phagocytosis of apoptotic cells through recognition of cell surface phosphatidylserine (5).

References

  1. Nishimori, H. et al. (1997) Oncogene 15:2145.
  2. Koh, J.T. et al. (2001) Brain Res. Mol. Brain Res. 87:223.
  3. Mori, K. et al. (2002) Neurosci. Res. 43:69.
  4. Kaur, B. et al. (2003) Am. J. Pathol. 162:19.
  5. Park, D. et al. (2007) Nature 450:430.
  6. Duda, D.G. et al. (2002) Br. J. Cancer 86:490.
  7. Lee, J.H. et al. (2001) Int. J. Oncol. 18:355.
  8. Fukushima, Y. et al. (1998) Int. J. Oncol. 13:967.
  9. Kaur, B. et al. (2005) Oncogene 24:3632.
  10. Koh, J.T. et al. (2004) Exp. Cell Res. 294:172.
  11. Kaur, B. et al. (2009) Cancer Res. 69:1212.
  12. Kang, X. et al. (2006) Cancer Gene Ther. 13:385.
  13. Hatanaka, H. et al. (2000) Int. J. Mol. Med. 5:181.

Long Name

Brain-specific Angiogenesis Inhibitor 1

Alternate Names

brain-specific angiogenesis inhibitor 1, FLJ41988, GDAIF

Entrez Gene IDs

575 (Human); 107831 (Mouse); 362931 (Rat)

Gene Symbol

ADGRB1

UniProt

O14514

Additional BAI1 Products

Product Documents for Human BAI1 Antibody

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Product Specific Notices for Human BAI1 Antibody

For research use only

Citations for Human BAI1 Antibody

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