Human Biglycan Antibody (303203)

R&D Systems | Catalog # MAB2667

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 303203
View all Biglycan Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Biglycan
Asp38-Lys368
Accession # P21810

Specificity

Detects human Biglycan in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Biglycan Antibody (303203)

Detection of Human Biglycan by Western Blot

Detection of Human Biglycan by Western Blot

The expression levels of hub genes in GC. (A) The mRNA expression was correlated with pathological T stages. The asterisk represents the comparison between a specific stage and the normal group, or between different stages. * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Representative western blotting for COL10A1, BGN, and FAP proteins in 4 paired gastric tumor (T) and adjacent non-tumor tissues (N) with progressive stages from T1 to T4. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35328635), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Biglycan Antibody (303203)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Biglycan (Catalog # 2667-CM)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Biglycan

Biglycan, also known as PG I, is a secreted chondroitin/dermatan sulfate proteoglycan in the small leucine-rich proteoglycan (SLRP) family. SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions that flank a central region containing 10 - 12 tandem leucine-rich repeats (LRRs). Biglycan function is important in the development and maintenance of many tissues (1, 2). The human Biglycan cDNA encodes a 368 amino acid (aa) precursor with a 19 aa signal sequence and a 18 aa propeptide that is cleaved by BMP-1 (3). Mature Biglycan contains N-linked glycosylation in addition to two glycosaminoglycan (GAG) chains (4). The 45 kDa core protein is approximately one third the molecular weight of the fully glycanated form and can assemble into noncovalently-associated dimers (5). Human Biglycan shares 97% aa sequence identity with bovine, mouse, and rat Biglycan and 57% aa sequence identity with human Decorin. Biglycan binds several matrix proteins, including fibrillar collagens, Matrilin-1, and beta IG-H3 (6 - 8). Its multiple LRRs, N-linked glycosylation, and GAG chains mediate distinct interactions that enable extensive crosslinking and stabilization of the collagen matrix (6 - 8). Mature Biglycan can be further cleaved by proteases, resulting in non-glycanated fragments (9). In osteoarthritis, MMP-13-induced breakdown of Biglycan likely interferes with collagen crosslinking and contributes to cartilage degradation (10). Biglycan also binds and modulates the activity of a variety of non-matrix proteins, including BMP-4, C1q, collectins, TGF-beta, TNF-alpha, and WISP-1 (11, 12). Biglycan functions as a proinflammatory mediator by binding TLR2 and TLR4 on macrophages and inducing TNF-alpha and MIP-2 production (13). Biglycan knockout mice have compromised inflammatory responses and are resistant to LPS-induced septic shock (13). Biglycan binds HDL/Apolipoprotein E complexes in atherosclerotic plaques and also binds SR-A, blocking the uptake and degradation of LDL (14, 15).

References

  1. Hocking, A.M. et al. (1998) Matrix Biol. 17:1.
  2. Yoon, J.H. and J. Halper (2005) J. Musculoskelet. Neuronal Interact. 5:22.
  3. Scott, I.C. et al. (2000) J. Biol. Chem. 275:30504.
  4. Fisher, L.W. et al. (1989) J. Biol. Chem. 264:4571.
  5. Scott, P.G. et al. (2006) J. Biol. Chem. 281:13324.
  6. Schonherr, E. et al. (1995) J. Biol. Chem. 270:2776.
  7. Wiberg, C. et al. (2003) J. Biol. Chem. 278:37698.
  8. Reinboth, B. et al. (2006) J. Biol. Chem. 281:7816.
  9. Roughley, P.J. et al. (1996) Biochem. J. 318:779.
  10. Monfort, J. et al. (2006) Arthritis Res. Ther. 8:R26.
  11. Groeneveld, T.W.L. et al. (2005) J. Immunol. 175:4715.
  12. Moreno, M. et al. (2005) EMBO J. 24:1397.
  13. Schaefer, L. et al. (2005) J. Clin. Invest. 115:2223.
  14. Olin, K.L. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:129.
  15. Santiago-Garcia, J. et al. (2003) J. Biol. Chem. 278:6942.

Alternate Names

BGN, DSPG1, PG-S1, SLRR1A

Entrez Gene IDs

633 (Human); 12111 (Mouse)

Gene Symbol

BGN

UniProt

P21810

Additional Biglycan Products

Product Documents for Human Biglycan Antibody (303203)

Certificate of Analysis

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Product Specific Notices for Human Biglycan Antibody (303203)

For research use only

Citations for Human Biglycan Antibody (303203)

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