Human Brevican Antibody (450613)

R&D Systems | Catalog # MAB4009

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Blockade of Receptor-ligand Interaction, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 450613
View all Brevican Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Brevican isoform 1
Asp23-Pro911
Accession # AAH09117

Specificity

Detects human Brevican in direct ELISAs and Western blots. Also detects a truncated version of recombinant human Brevican (aa 23‑649).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Brevican Antibody (450613)

Blockade of Receptor-ligand Interaction by Human Brevican Antibody.

Human Brevican Antibody (Catalog # MAB4009) blocks the binding of biotinylated Hyaluronan to Recombinant Human Brevican (4009-BC). The Neutralization Dose (ND50) is typically 0.0200-0.400 µg/mL.

Applications for Human Brevican Antibody (450613)

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

Measured by its ability to block the binding of biotinylated Hyaluronan to Recombinant Human Brevican (Catalog # 4009-BC). The Neutralization Dose (ND50) is typically 0.0200-0.400 µg/mL.

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Brevican (Catalog # 4009-BC), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Brevican (Catalog # 4009-BC) under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Brevican

Brevican, also called BEHAB, is a secreted member of the the lectican family of proteoglycans that share a common domain structure (1). Brevican contains an Ig‑like V-set domain, two link domains, a Glu-rich region, a central region with glycosaminoglycan (GAG) modifications, an EGF-like domain, a C-type lectin domain, and a C-terminal Sushi/CRP-like domain (2). Brevican is restricted to the CNS and is expressed by astrocytes, oligodendrocytes, and neurons (3‑7). A GPI-anchored alternate splice form exists that is truncated following the central (GAG) region (2, 8). An additional isoform results from an alternate start site at Met228. Brevican is cleaved by multiple proteases and exists in a number of distinct fragments (5, 9, 10). Full-length brevican consists of a 97 kDa core protein with up to approximately 100 kDa of attached chondroitin sulfate but not heparan sulfate chains (4, 7, 11, 12). Brevican associates with the extracellular matrix, perineuronal nets, and astrocyte cell surfaces by means of its chondroitin sulfate, GPI anchor, hyaluronic acid-binding link domains, and the core protein (4, 7, 8, 13). The secreted isoform is dominant during brain development and is upregulated in astrocytes following brain injury (2, 14). In human and rat, an under-glycosylated form of brevican is upregulated in highly aggressive glioma but not in low-grade glioma or other brain pathologies (15, 16). In mouse and rat, levels of an ADAMTS4-generated 55 kDa N‑terminal fragment increase during remodeling after excitotoxic injury (11, 12). Human brevican shares 90%, 80%, and 80% aa sequence identity with bovine, mouse, and rat brevican, respectively. Within the Ig‑like and two link domains, brevican shares 45%‑51% aa sequence identity with aggrecan, neurocan, and versican.

References

  1. Viapiano, M.S. and R.T. Matthews (2006) Trends Mol. Med. 12:488. 
  2. Gary, S.C. et al. (2000) Gene 256:139. 
  3. Jaworski, D.M. et al. (1994) J. Cell Biol. 125:495. 
  4. Seidenbecher, C.I. et al. (1995) J. Biol. Chem. 270:27206. 
  5. Hamel, M.G. et al. (2005) J. Neurochem. 93:1533. 
  6. Ogawa, T. et al. (2001) J. Comp. Neurol. 432:285  
  7. Yamada, H. et al. (1997) J. Neurosci. 17:7784.  
  8. Seidenbecher, C.I. et al. (2002) J. Neurochem. 83:738.
  9. Matthews, R.T. et al. (2000) J. Biol. Chem. 275:22695.
  10. Nakamura, H. et al. (2000) J. Biol. Chem. 275:38885.
  11. Mayer, J. et al. (2005) BMC Neurosci. 6:52.
  12. Yuan, W. et al. (2002) Neuroscience 114:1091.
  13. Deepa, S.S. et al. (2006) J. Biol. Chem. 281:17789.
  14. Jaworski, D.M. et al. (1999) Exp. Neurol. 157:327.
  15. Viapiano, M.S. et al. (2005) Cancer Res. 65:6726.
  16. Viapiano, M.S. et al. (2003) J. Biol. Chem. 278:33239.

Long Name

Brain Specific Proteoglycan in the Aggrecan Family

Alternate Names

ALPBRE, BCAN, BEHAB, CSPG7

Entrez Gene IDs

63827 (Human); 12032 (Mouse); 25393 (Rat)

Gene Symbol

BCAN

UniProt

AAH09117

Additional Brevican Products

Product Documents for Human Brevican Antibody (450613)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Brevican Antibody (450613)

For research use only

Citations for Human Brevican Antibody (450613)

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