Human Carbonic Anhydrase I/CA1 Antibody

Catalog #: MAB2180 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2180
MAB2180-SP

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Detection of Human Carbonic Anhydrase I/CA1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase I/CA1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbonic Anhydrase I/CA1 in direct ELISAs and Western blots. In Western blots, 30% cross-reactivity with recombinant human (rh) CA2 is observed and no cross-reactivity with rhCA3, -4, -7, -8, -10, -11, -12, -13, or -14 is observed.

Source

Monoclonal Rat IgG_2A Clone # 363121

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase I/CA1
Ala2-Phe261
Accession # P00915

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Carbonic Anhydrase I/CA1 by Western Blot. Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, human liver tissue, and human colon tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Carbonic Anhydrase I/CA1 Monoclonal Antibody (Catalog # MAB2180) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase I/CA1

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). CA1 is a cytosolic enzyme with the highest levels in erythrocytes and is a very early marker for erythroid differentiation (2). The activity of CA1 can also be measured by its ability to catalyze the reaction CO₂ + H₂O → HCO₃^- + H⁺, using a published method (3).

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.
Wilbur, K.M. and N.G. Anderson (1948) J. Biol. Chem. 176:147.

Entrez Gene IDs

759 (Human)

Alternate Names

CA1; CA-I; Car1; Carbonate dehydratase I; carbonic anhydrase 1; Carbonic anhydrase B; Carbonic Anhydrase I; carbonic anhydrase ICAB; carbonic dehydratase; EC 4.2.1.1