Human CARF Alexa Fluor® 532-conjugated Antibody

Catalog #: AF4195X Datasheet / COA / SDS

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AF4195X-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CARF Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CARF in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CARF
Ser379-Leu579
Accession # Q9NXV6

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CARF

CARF (collaborator of ARF) is a component of the ARF-MDM2-p53 pathway. CARF interacts with ARF in the nucleolus accentuating ARF-mediated p53 stabilization and activation. Data also suggests that in the absence of ARF, CARF interacts directly with p53 in the nucleoplasm resulting in a moderate stabilization and activation of p53.

Long Name

Collaborates/Cooperates With ARF [Alternate Reading Frame] Protein

Entrez Gene IDs

55602 (Human); 70925 (Mouse)

Alternate Names

CARF; CARFCDKN2A-interacting protein; CDKN2A interacting protein; CDKN2AIP; collaborates/cooperates with ARF (alternate reading frame) protein; Collaborator of ARF; FLJ20036