Human Chitobiase/CTBS Alexa Fluor® 350-conjugated Antibody

Catalog #: AF4829U Datasheet / COA / SDS

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AF4829U-100UG

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Human Chitobiase/CTBS Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Chitobiase/CTBS in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chitobiase/CTBS
Thr39-Arg385
Accession # Q01459

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Chitobiase/CTBS

CTBS (Di-N-acetylchitobiase; also chitobiase) is a 40 kDa lysosomal glycoprotein that belongs to glycosyl hydrolase family # 18. It is a reducing-end glycoside hydrolase that serves to degrade asparagines-linked carbohydrates. Mature human CTBS is 347 amino acids (aa) in length and contains one catalytic domain (aa 115‑357). There are multiple potential isoforms of CTBS. One shows a three aa deletion of aa 30‑32, accompanied by a two two aa substitution for the C-terminal 279 amino acids. There are two potential alternate start sites, one at Met92 and a second at Met207. Mature human CTBS (aa 39‑385) is 85% aa identical to mouse CTBS.

Entrez Gene IDs

1486 (Human); 74245 (Mouse); 81652 (Rat)

Alternate Names

Chitobiase; chitobiase, di-N-acetyl-; CTBdi-N-acetylchitobiase; CTBS; EC 3.2.1.-