Human CIS-1 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB3194R Datasheet / COA / SDS

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FAB3194R-100UG

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Human CIS-1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CIS-1 in direct ELISAs.

Source

Monoclonal Rat IgG2a Clone # 989616

Purification

Protein A or G purified

Immunogen

E.coli-derived recombinant human CIS-1
Leu11-Leu258
Accession # Q9NSE2

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CIS-1

Cytokine Inducible SH2-containing protein (CIS-1) is a 29 kDa protein found in a variety of cell types. Mono or polyubiquitination generally results in a 37 or 45 kDa molecule. CIS-1 binds to phosphorylated cytokine receptors IL-3 R beta and EPO-R and blocks downstream activation of STAT5 via receptor internalization and ubiquitin‑mediated proteosomal degradation. Human CIS-1 is a 258 aa peptide that contains one SH2 domain (aa 82‑163) and one SOCS box (aa 218‑258). There are two known alternatively spliced variants with a 7- or 13-aa substitution for the 7 N-terminal amino acid residues. Over the region used as immunogen, human CIS-1 is 91% identical to the corresponding mouse and canine protein sequences.

Long Name

Cytokine Inducible SH2-containing Protein

Entrez Gene IDs

1154 (Human); 12700 (Mouse); 83681 (Rat)

Alternate Names

CIS-1; CIS-1cytokine-inducible SH2-containing protein; CIScytokine-inducible inhibitor of signaling type 1B; CISH; cytokine inducible SH2-containing protein; G18Protein G18; SOCS; Suppressor of cytokine signaling