Key Product Details

Species Reactivity

Validated:

Human

Cited:

Bacteria - Bordetella pertussis

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Complement Component C1r Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Component C1r
Ser18-Asp705
Accession # NP_001724

Specificity

Detects human Complement Component C1r in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Complement Component C1s is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Complement Component C1r Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Component C1r (Catalog # 1807-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Complement Component C1r (Catalog # 1807-SE)

Reviewed Applications

Read 2 reviews rated 4 using AF1807 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Component C1r

The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates (1). Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B) (2). The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. The B chains contain the serine protease catalytic domain. The full-length (amino acid residues 1‑705) of human C1r was expressed, which had the Leu152 natural variant (3). The purified protein corresponded to the processed active form, with A and B chains starting at residue Ser18 and Ile464, respectively.

References

  1. Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.
  2. Lacroix, M. et al. (2001) J. Biol. Chem. 276:36233.
  3. Journet A. and M. Tosi (1986) Biochem. J. 240:783.

Alternate Names

C1r

Entrez Gene IDs

715 (Human)

Gene Symbol

C1R

UniProt

NP_001724

Additional Complement Component C1r Products

Product Documents for Human Complement Component C1r Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Complement Component C1r Antibody

For research use only

Citations for Human Complement Component C1r Antibody

Customer Reviews for Human Complement Component C1r Antibody (2)

4 out of 5
2 Customer Ratings
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  • Human Complement Component C1r Antibody
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 10/12/2019
    Human Complement Component C1r Antibody AF1807
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 21584940
    Species: Human
    Verified Customer | Posted 01/05/2015

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