Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Flow Cytometry, Zymography

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Complement Component C1s Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Component C1s
Glu16-Asp688
Accession # P09871

Specificity

Detects human Complement Component C1s in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than2% cross‑reactivity with recombinant human C1r is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Complement Component C1s Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Complement Component C1s (Catalog # 2060-SE)

Reviewed Applications

Read 1 review rated 4 using AF2060 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Component C1s

The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates (1). Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B) (2). The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. The B chains contain the serine protease catalytic domain. The full-length (amino acid residues 1‑688) of human C1s was expressed (3‑5). The purified protein corresponded to the processed active form, with A and B chains starting at residue Glu16 and Ile438, respectively.

References

  1. Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.
  2. Lacroix, M. et al. (2001) J. Biol. Chem. 276:36233.
  3. Tosi, M. et al. (1987) Biochemistry 26:8516.
  4. Mackinnon, C.M. et al. (1987) Eur. J. Biochem. 169:547.
  5. Kusumoto, H. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7307.

Alternate Names

C1S

Entrez Gene IDs

716 (Human)

Gene Symbol

C1S

UniProt

P09871

Additional Complement Component C1s Products

Product Documents for Human Complement Component C1s Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Complement Component C1s Antibody

For research use only

Citations for Human Complement Component C1s Antibody

Customer Reviews for Human Complement Component C1s Antibody (1)

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  • Human Complement Component C1s Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 12/08/2018
    Human Complement Component C1s Antibody AF2060

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