Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Complement Component C9 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived human Complement Component C9
Gln22-Lys559 (predicted)
Accession # P02748

Specificity

Detects human Complement Component C9 in directs ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Complement Component C9 Antibody

Detection of Human Complement Component C9 antibody by Western Blot.

Detection of Human Complement Component C9 by Western Blot.

Western blot shows lysates of human plasma. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Complement Component C9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8126) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Component C9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Complement Component C9 Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: Human plasma

Reviewed Applications

Read 1 review rated 5 using AF8126 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Component C9

Complement Component 9 (C9) is a 65-70 kDa protein of the complement membrane attack complex (C5b-9) that binds C5b-8, polymerizes, and forms pores upon activation of the complement cascade. Mature human C9 shares 60% and 66% amino acid sequence identity with mouse and rat C9, respectively. It may be proteolytically cleaved by thrombin into a 28-34 kDa cysteine-rich, mannosylated soluble C9a subunit, and a 37-38 kDa membrane-anchored N-glycosylated C9b. These fragments are also able to polymerize.

Alternate Names

complement component 9, complement component C9

Entrez Gene IDs

735 (Human); 12279 (Mouse); 117512 (Rat)

Gene Symbol

C9

UniProt

P02748

Additional Complement Component C9 Products

Product Documents for Human Complement Component C9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Complement Component C9 Antibody

For research use only

Customer Reviews for Human Complement Component C9 Antibody (1)

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  • Human Complement Component C9 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: serum
    Verified Customer | Posted 04/17/2024
    Human Complement Component C9 Antibody AF8126

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