Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 556317
View all Complement Factor H Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Factor H
Ser860-Arg1231
Accession # P08603

Specificity

Detects human Complement Factor H in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Complement Factor H Antibody

Detection of Human Complement Factor H antibody by Western Blot.

Detection of Human Complement Factor H by Western Blot.

Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human Complement Factor H Monoclonal Antibody (Catalog # MAB4779) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Complement Factor H at approximately 155 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Complement Factor H Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Factor H, see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: Human liver tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor H

Complement Factor H (CFH) is a 155 kDa glycoprotein that negatively regulates the alternative pathway complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets and circulates in the serum at high concentration. CFH interacts with cell surface polyanions and prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5, and functioning as a cofactor for Factor I. CFH is composed of 20 SCR (short consensus repeats). Alternate splicing generates an isoform that is truncated following SCR7. Within SCR5-20 (aa 260-1231), human CFH shares 60% and 62% aa sequence identity with mouse and rat CFH, respectively.

Alternate Names

ARMD4, ARMS1, beta-1-H-globulin, CFH, CFHL3, FH, FHL1, HF1, HF2, HUS

Entrez Gene IDs

3075 (Human); 12628 (Mouse); 155012 (Rat)

Gene Symbol

CFH

UniProt

P08603

Additional Complement Factor H Products

Product Documents for Human Complement Factor H Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Complement Factor H Antibody

For research use only

Citations for Human Complement Factor H Antibody

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