Human/Cynomolgus Monkey IL-23R Antibody

Catalog #: MAB106242 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB106242-100
MAB106242-SP

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Detection of Human IL-23R by Western Blot.

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Human/Cynomolgus Monkey IL-23R Antibody Summary

Species Reactivity

Human, Cynomolgus Monkey

Specificity

Detects cynomolgus monkey IL-23R in direct ELISAs and human IL-23R in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 1032638

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived cynomolgus monkey IL-23R
Gly24-Asp353
Accession # XP_005543141.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

K562 human chronic myelogenous leukemia cell line, MCF‑7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL-23R by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MCF‑7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Cynomolgus Monkey IL-23R Monoclonal Antibody (Catalog # MAB106242) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for IL-23R at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12R beta 1) and the IL-23-specific receptor subunit (IL-23R) (3). Human IL-23R cDNA encodes a 629 amino acids (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12R beta 2. IL-23R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif (6). The cytoplasmic region of IL-23R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23R is located on human chromosome 1 within 150 kb of IL-12R beta 2. Based on quantitative real-time PCR, human IL-23R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5 (2). The Cynomolgus IL-23R shares 96%, 71% and 77% amino acid sequence identity to Human, mouse, and rat IL-23R, respectively.

References