Human Cystatin A Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB1407G Datasheet / COA / SDS

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FAB1407G-100UG

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All Cystatin A Antibodies

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Human Cystatin A Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cystatin A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Cystatins B, C, D, E/M, F, S, SA, rhFetuin A, rhFetuin B, rhHPRG, rhKininogen, or rhKininostatin is observed.

Source

Monoclonal Mouse IgG2a Clone # 224705

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Cystatin A
Ile2-Phe98
Accession # P01040

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cystatin A

Cystatin A, also called stefin A, Cystatin AS or keratolinin, is a member of family 1 of the cystatin superfamily (1, 2). Like Cystatin B, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (3). For example, immunohistochemical analysis of Cystatin A and cathepsin L is a useful indicator for malignancy in human epidermal keratinocytes (4). The ratio of cathepsin B and Cystatin A can be used in the differential diagnosis and treatment of patients with prostate carcinoma (5). The human Cystatin A consists of 98 amino acids (1).

Entrez Gene IDs

1475 (Human)

Alternate Names

AREI; CSTA; cystatin A (stefin A); Cystatin A; cystatin AS; cystatin-A; Stefin A; STF1; STF1Cystatin-AS; STFA; STFAStefin-A